Nucleic Acid Purity Assessment using A260/A280 Ratios
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چکیده
A common practice in molecular biology is to perform a quick assessment of the purity of nucleic acid samples by determining the ratio of spectrophotometric absorbance of the sample at 260 nm to that of 280 nm. Here we describe the basis of A260/A280 ratio determinations of nucleic acids and demonstrate the utility of the BioTek PowerWaveTM 200 scanning microplate spectrophotometer to perform this procedure on large numbers of samples.
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Nucleic Acid Purity Assessment using A260/A280 Ratios
A common practice in molecular biology is to perform a quick assessment of the purity of nucleic acid samples by determining the ratio of spectrophotometric absorbance of the sample at 260 nm to that of 280 nm. Here we describe the basis of A260/A280 ratio determinations of nucleic acids and demonstrate the utility of the BioTek PowerWaveTM 200 scanning microplate spectrophotometer to perform t...
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